Description:

Mouse monoclonal antibody

  Background:

Phytochromes (PHYs) and cryptochromes (CRYs) are two major classes of photoreceptors which control over similar aspects of plant development such as de-etiolation, plant architecture and flowering. In Arabidopsis, there are five PHY isoforms: A (AT1G09570), B (AT2G18790), C (AT5G35840), D (AT4G16250) and E (AT4G18130). PHYA protein accumulates to a high level in etiolated seedlings and acts primarily as a far-red sensor at the stages of early seedling development.

  Synonyms:

PHYA, ELONGATED HYPOCOTYL 8, FAR RED ELONGATED 1, FAR RED ELONGATED HYPOCOTYL 2, FHY2, FRE1, HY8, PHYTOCHROME A

  Immunogen:

Recombinant protein of PHYA derived from Arabidopsis thaliana AT1G09570.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000); 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

125 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

Electrophoresis: 8% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006)
Detection: using chemiluminescence substrate and image were captured with CCD camera.