Sigma(ESG1106)ESGRO®重组小鼠LIF蛋白

国产进口 : 进口
产地品牌 : 美国/Sigma
型号 : 1000000units

总访问 : 2577
产品类别 : 蛋白质组
最后更新 : 2026-02-06 09:31:47

产品介绍

公司简介

名称	品牌	货号	规格	保存
ESGRO®重组小鼠LIF蛋白 ESGRO® Recombinant Mouse LIF Protein	Sigma	ESG1106	1000000units	4℃

一、产品概述
ESGRO小鼠LIF培养基补充剂以有效单位/mL的形式提供，以确保批次之间的性能一致。每批ESGRO补充剂均经过严格的质量控制测试，以确保能可靠地抑制小鼠多能干细胞分化。ESGRO小鼠LIF补充剂可用于小鼠胚胎干(ES)和诱导性多能干(iPS)细胞的基于饲养层和无饲养层的培养。
小鼠白血病抑制因子(LIF,mLIF)通过抑制自发分化，促进多能小鼠胚胎干(ES)和诱导多能干(iPS)细胞的自我更新和长期维持。

二、产品特点
• 一致的ES细胞分化抑制，获得可靠的效果。
• 无批间差异，获得更佳的重复性。
• 高度灵活，支持无饲养层和基于饲养层的细胞培养。
• 方便的包装规格，以活性单位/毫升形式提供。

三、产品应用
•ESGRO白血病抑制因子(LIF)补充剂，用于小鼠ES细胞培养。每小瓶含有10^6单位/ml
•ESGRO补充剂的应用包括用作体外维持鼠 ES 细胞多能表型的试剂。
建议的实验方案：
•ES细胞：在D3和MBL-1多能ES细胞培养物中，研究发现，通常每1.0mL组织培养基需要1000 U ESGRO补充剂来维持具有干细胞表型的ES细胞。相似浓度的mLIF也已用于遗传修饰的ES细胞的种系传递(E3)。
•在推荐浓度下,107单位的ESGRO补充剂足以用于10.0 L的组织培养基，而106单位的ESGRO补充剂足以用于1.0 L的组织培养基。

四、外形
ESGRO补充剂以液体形式提供，含量为106单位，溶于1.0mL磷酸盐缓冲盐水，含1％w/v牛血清白蛋白BSA为载体以确保稳定性。
形式：纯化

五、制备说明
ESGRO 补充剂在环境温度下运输。出于客户关系，可能包含一个冰袋，但确实没必要。开发期间已使用 ESGRO 补充剂进行了广泛的稳定性测试。该产品自生产之日起，以浓缩形式或在无菌组织培养基中稀释后至少可稳定保存 18 个月，且对 ES 细胞没有活性损失。若要长期储存，建议将 ESGRO 补充剂储存于 4°C。冻融会降低效力。建议在使用前，将 ESGRO 补充剂在无菌组织培养基中稀释并等分至合适的浓度，然后储存于 4°C。应避免冻融。在 ES 细胞培养期间，该产品在 37°C，5％ CO2 培养箱中可稳定至少 7 天。
提供的 ESGRO® 补充剂经 0.22 微米无菌过滤，并在支原体检测中呈阴性。

六、分析说明
比活度：ESGRO® 补充剂在小鼠胚胎干细胞（D3 & MBL-1）和鼠 M1 髓样白血病细胞上进行了评估（4）。50 单位的标准定义为在 1.0mL 组织培养基中可诱导 50％的 M1 菌落分化的 ESGRO 补充剂的浓度（4）。 106 单位相当于大约 10 μg 的纯蛋白质，足以处理 1L 的 ES 细胞培养基。
胚胎干细胞测定：分化抑制测定，浓度为 1000 单位/mL 小鼠骨髓白血病细胞 M1：比活度 >/= 108 单位/mg

七、产品属性

质量水平	100
方案	>95% (active component mLIF, SDS-PAGE)
表单	liquid
制造商/商品名称	Chemicon®
浓度	1000000 units/mL
技术	cell culture \| stem cell: suitable
输入	sample type: mouse embryonic stem cell(s) sample type induced pluripotent stem cell(s)
NCBI登记号	NM_008501
UniProt登记号	P09056
运输	wet ice
基因信息	mouse ... LIF(16878)

八、运输与保存
湿冰运输；4℃保存

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